Objective: To observe and analyze the effects of humanistic care interventions on anxiety and satisfaction among operating room patients. Methods: A total of 88 patients undergoing elective surgery at Pingdingshan First People’s Hospital were selected between April 2024 and April 2026 and assigned to groups using a random number table. The control group (standard nursing care) consisted of 44 patients, and the observation group (humanistic care intervention in addition to standard nursing care) consisted of 44 patients. Anxiety levels, satisfaction with nursing care, and the incidence of adverse stress events during surgery were compared between the two groups. Results: Compared with the control group, the intervention group exhibited significantly lower levels of anxiety and depression after the intervention (P < 0.05); the intervention group reported significantly higher overall satisfaction with nursing care than the control group (P < 0.05); and the intervention group had a significantly lower incidence of adverse stress events during surgery than the control group (P < 0.05). Conclusion: Implementing humanistic care interventions for patients undergoing elective surgery in the operating room can significantly reduce patients’ anxiety scores, decrease adverse stress events such as intraoperative blood pressure fluctuations and limb agitation, and enhance patients’ satisfaction with operating room nursing care; this approach is worthy of wider implementation.