Objective: To explore the correlation between lifestyle and behavioral factors and the risk of bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). Methods: A total of 150 gynecological patients admitted from May 2023 to May 2025 were selected and divided into the BV group, the VVC group and the control group (50 cases in each group) according to the diagnosis results. Lifestyle and behavioral factors were collected through questionnaires, and multivariate Logistic regression analysis was used to analyze the correlation between lifestyle and behavioral factors and the risk of BV and VVC. Results: Univariate analysis showed that there were statistically significant differences in menstrual cycle, frequency of changing menstrual hygiene products, frequency of vaginal cleaning, frequency of sexual life, number of sexual partners, contraceptive methods, dietary preferences, and sleep quality between the BV group and the VVC group and the control group (P<0.05). Multivariate Logistic regression showed that irregular menstrual cycle, frequency of changing menstrual hygiene products <3 times/day, frequency of vaginal cleaning ≥2 times/week, frequency of sexual life ≥3 times/week, number of sexual partners ≥2, external ejaculation OR no contraceptive measures, preference for sweet or spicy food, and poor sleep quality (all OR>1) P<0.05) were all risk factors for the onset of BV and VVC. Conclusion: Lifestyle and behavioral factors are closely related to the risk of BV and VVC. Clinical intervention in women"s lifestyle and behavior should be strengthened to reduce the risk of BV and VVC.