Objective: To investigate the clinical value of transabdominal ultrasonography combined with high-frequency ultrasonography in the diagnosis of gallbladder polyposis. Methods: From March 2022 to March 2024, 98 patients with suspected gallbladder polyps were enrolled in Xinzheng Public People"s Hospital. After admission, all patients were diagnosed by abdominal ultrasonography and high-frequency ultrasonography. According to the pathological examination results, the diagnostic efficiency of ultrasonography was calculated and the ultrasonic signs of gallbladder polyps were analyzed. Results: Pathological examination confirmed 91 benign gallbladder polyps, of which 52 (53.06%) were cholesterol polyps, 20 (20.41%) inflammatory polyps, 11 (11.22%) gallstones, 8 (8.16%) adenomatous polyps, and 7 malignant gallbladder polyps were gallbladder cancer, accounting for 7.14% (7/98). The accuracy, sensitivity and specificity of single transabdominal ultrasonography were 85.71%, 87.91% and 57.14%, and the Kappa agreement coefficient was 0.585. The accuracy, sensitivity and specificity of single high-frequency ultrasonography were 94.90%, 96.70% and 71.43%, respectively, and the Kappa consistency coefficient with pathological examination results was 0.602. The accuracy, sensitivity and specificity of transabdominal ultrasonography combined with high-frequency ultrasonography were 96.94%, 97.80% and 85.71%, and the Kappa consistency coefficient was 0.899. The diameter, blood flow signal, basal morphology width and single lesion of malignant gallbladder polyp were significantly higher than those of benign gallbladder polyp lesion (P<0.05). Conclusion: The combined application of transabdominal ultrasound and high-frequency ultrasound is more effective in the clinical diagnosis of patients with gallbladder polyp lesions, which is helpful to accurately distinguish the good and bad properties of gallbladder polyp lesions and provide evidence-based support for clinical symptomatic management.