Objective: To evaluate the effects of malformation rate and DNA fragmentation index on the development of assisted reproductive technology embryos and pregnancy outcomes. Method: This study is a retrospective analysis of 108 couples who underwent assisted reproductive technology in our hospital from January 2022 to January 2024. The cycles were divided into two groups based on the male"s DNA level: Group A (DFI<25%) (79 cases) and Group B (DFI ≥ 25%) (29 cases). The fertilization rate, excellent embryo rate, clinical pregnancy rate, and early miscarriage rate were compared between the two groups. At the same time, based on the analysis of sperm morphology, compare the pregnancy outcomes between the abnormal sperm group and the normal morphology sperm group. Result: The fertilization rate, cleavage rate, and high-quality embryo rate in Group A were higher than those in Group B (P<0.05); The early miscarriage rate in Group A was lower than that in Group B (P<0.05), while the pregnancy rate and sustained pregnancy rate were higher than those in Group B (P<0.05); The pregnancy rate of the group with normal sperm morphology was higher than that of the group with abnormal sperm morphology (P<0.05). Conclusion: High levels of sperm DNA fragmentation index and abnormality rate are the main influencing factors for adverse pregnancy outcomes of assisted reproductive technology. For couples planning to undergo assisted reproductive technology, it is important to pay attention to the detection of male sperm deformity rate and DNA fragmentation index, which can facilitate early personalized intervention and improve prognosis.