Objective: To explore the value of gamma glutamyl transfer (GGT), cystatin C (CYS-C) and lactate dehydrogenase (LDH) in the early diagnosis of type 2 diabetes (T2MD). Methods: A total of 100 newly diagnosed T2MD patients admitted to our hospital from January 2022 to June 2025 were selected as the T2MD group, and another 100 healthy individuals who underwent physical examinations during the same period were selected as the control group. Fasting venous blood was collected in the morning to detect the expression levels of glycated hemoglobin (HbAlc), GGT, CYS-C, and LDH, and the early diagnostic value of GGT, CYS-C, and LDH for T2MD was analyzed. Results: Comparison of laboratory indicators showed that the levels of HbAlc, GGT, CYS-C, and LDH in the T2MD group were significantly higher than those in the control group (P<0.05); As the severity of T2MD patients increases, the levels of GGT, CYS-C, and LDH significantly increase, with severe patients showing significantly higher levels than mild and moderate patients (P<0.05); The Pearson correlation analysis results showed that GGT, CYS-C, and LDH were significantly positively correlated with HbAlc (all P<0.05); ROC curve analysis showed that the optimal thresholds for GGT, CYS-C, and LDH in diagnosing T2MD were 36.50U ? L-1, 1.12mg ? L-1, and 192.45 U ? L-1, respectively. The combined detection of the three indicators had the best diagnostic performance, AUC=0.833, with sensitivity and specificity of 76.79% and 86.74%, respectively, significantly better than single indicator detection. Conclusion: The combined detection of GGT, CYS-C, and LDH has ideal application value in the early diagnosis of T2MD.