Objective: To analyze the impact of mycoplasma infection (GMI) of the female reproductive tract on pregnancy. Methods: A total of 200 pregnant women in a hospital were studied and divided into the infected group and the non-infected group according to whether they developed GMI. Among them, the infected group received conventional antibacterial treatment. Analyze the GMI conditions of the observed subjects and compare the delivery methods and pregnancy outcomes of the two groups. Results: Among 200 pregnant women, a total of 78 cases were infected with mycoplasma of the reproductive tract, with an infection rate of 39.00%. The cesarean section rate (60.26%) and vaginal delivery rate (19.23%) in the infection group were higher than those in the non-infection group (27.05%, 9.02%), and the natural delivery rate (20.51%) was lower than that in the non-infection group (63.93%), P < 0.05; The incidences of preterm birth, premature rupture of membranes, puerperal infection and placental adhesion in the infected pregnant women were higher than those in the non-infected group, P < 0.05. The incidences of neonatal pneumonia, low birth weight infants, bilirubinemia and respiratory distress syndrome in the infection group were higher than those in the control group, P < 0.05. Conclusion: The incidence of GMI is relatively high among pregnant women, and the main type of infection is Ureaplasma urealyticum. After the infection occurs, it significantly increases the clinical cesarean section rate and the risk of adverse perinatal outcomes.