Objective: To explore the predictive value of combined detection of hypersensitive troponin Ⅰ (TnI-Ultra) and myoglobin (Myo) in serum for the short-term prognosis of patients with high-risk non-ST-segment elevation myocardial infarction (NSTEMI) after percutaneous coronary intervention (PCI). Methods: Eighty patients with NSTEMI who underwent PCI surgery in Yingtan People"s Hospital from January to December 2023 were selected and divided into the good prognosis group (n=57) and the poor prognosis group (n=23) according to the short-term prognosis. Univariate and multivariate Logistic regression analyses were used to analyze the influencing factors of the short-term prognosis of NSTEMI patients after PCI. The receiver operating characteristic (ROC) curve was used to analyze the levels of TnI-Ultra and Myo and the combined detection for predicting the short-term prognosis of patients with NSTEMI after PCI. Results: The number of lesion blood vessels, LVEF, and the levels of TnI-Ultra and Myo in serum are influencing factors for the short-term prognosis of patients with NSTEMI after PCI (P < 0.05); Multivariate results showed that a large number of lesion blood vessels, low LVEF, and high levels of TnI-Ultra and Myo in serum were independent influencing factors affecting the short-term prognosis of NSTEMI patients after PCI (P < 0.05); The results of the ROC curve showed that the areas under the curves of TnI-Ultra, Myo and their combined detection in serum were 0.853, 0.847 and 0.928 respectively (P < 0.05). Conclusion: The levels of TnI-Ultra and Myo in serum have predictive value for the short-term prognosis of NSTEMI patients after PCI, and the combined predictive value of TnI-Ultra and Myo is higher.