Abstract Objective: To evaluate the value of Fine-needle aspiration biopsy (FNAB) combined with Thyroglobulin (FNA-TG) in cervical lymph node metastasis after thyroid cancer surgery. Methods: From September 2022 to August 2024, 68 patients with suspected cervical lymph node metastasis after thyroid cancer surgery in our hospital were selected as the study objects. FNAB and FNA-TG were detected in suspicious cervical lymph nodes. The surgical and pathological results are used as the gold standard to determine whether lymph node metastasis is positive. To analyze the diagnostic efficacy of FNAB, FNA-TG and their combined detection in cervical lymph node metastasis. Results: The pathological results of 68 patients showed cervical lymph node metastasis in 42 cases and no metastasis in 26 cases. There were 28, 30 and 38 true positive cases, 4, 5 and 2 false positive cases, 22, 21 and 24 true negative cases, and 14, 12 and 4 false negative cases diagnosed by FNAB and FNA-TG alone and in combination, respectively. The sensitivity, accuracy and positive predictive value of FNAB combined with FNA-TG detection were 90.48%, 91.17% and 95.00%, which were significantly higher than those of FNAB alone (66.67%, 73.53%, 87.50%) or FNA-TG detection alone (71.43%, 75.00%). 85.71%) (P<0.05). Conclusion: FNAB combined with FNA-TG can improve the diagnostic accuracy of cervical lymph node metastasis after thyroid cancer surgery.