Objective:? Analysis of rapid puncture sampling versus normal speed puncture sampling in the satisfaction rate of thyroid fine-needle cytology specimen sampling. Methods:? One hundred and twenty patients with thyroid nodules (TN) diagnosed and treated in Pingxiang People"s Hospital from March 2023 to February 2025 were selected. According to different conditions, TN was divided into three subgroups: with or without calcification group, different echo group, and blood supply group, and each subgroup was sampled by different speeds of puncture, which were respectively the observation group (fast puncture sampling, puncture time <3s) and the control group (general speed puncture sampling, puncture time >3s), with 60 cases in each group. Comparison of patients in the observation group and the control group in terms of satisfaction rate, complication rate of puncture, the degree of pain of puncture, and the degree of pain of puncture. Results:? The satisfaction rate of thyroid cytopathology Bethesda Reporting System for Thyroid Cytopathology (TBSRTC) sampling was significantly higher in the observation group (91.67%) than in the control group (75.00%)(P<0.05); the incidence of post-puncture pain and discomfort (1.67% vs 5.00%), infection (3.33%vs5.00%), dysphagia (1.67% vs 6.67%), and postoperative hematoma (1.67% vs 5.00%) was lower in the observation group than in the control group (21.67%)(P<0.05); and the Pain Numeric Ranging Score (NRS) was significantly lower in the observation group than in the control group (P<0.05). Conclusion:? The use of rapid puncture sampling under ultrasound guidance can effectively improve the satisfaction of sampling, reduce rate of puncture complications, reduce the patient"s pain, and is worthy of clinical promotion and application.