Objective: To explore the effects of different load doses of dexmedetomidine pre-injection on anesthetics dosage and recovery quality during laparoscopic cholecystectomy. Methods: A total of 72 patients undergoing laparoscopic cholecystectomy in the hospital were enrolled as the research objects between June 2023 and June 2024. According to different load doses of dexmedetomidine pre-injection, patients were divided into group A (0.5μg/kg dexmedetomidine, n=24), group B (1.0μg/kg dexmedetomidine, n=24) and group C (the same volume of 0.9% sodium chloride solution, n=24). The perioperative indexes (dosages of remifentanil and propofol, operation time), hemodynamics [mean arterial pressure (MAP), heart rate (HR)] and recovery quality were compared among the three groups. Results: The dosages of remifentanil and propofol were lower in group B than groups A and C, difference in remifentanil dosage and operation time between group A and group C was not statistically significant (P>0.05), and propofol dosage was lower in group B groups A and C (P<0.05). At T1, difference in MAP and HR among the three groups was not statistically significant (P>0.05). At T2, T3 and T4, difference in MAP and HR between group A and group B was not statistically significant (P>0.05), and fluctuations of MAP and HR were lower in group B than groups A and C (P<0.05). The difference in the quality of anesthesia recovery (respiratory recovery time, orientation recovery time, eye opening time) between group A and group B was not statistically significant (P>0.05), but the above three indexes were longer in group C than groups A and B (P<0.05). Conclusion: Pre-injection of 1.0μg/kg dexmedetomidine can reduce dosages of anesthetics, stabilize hemodynamic fluctuation and improve recovery quality in patients undergoing laparoscopic cholecystectomy.