Objective: Exploring the application value of low ion condensed amine technology in traumatic blood transfusion testing. Methods: 102 patients with traumatic blood loss requiring blood transfusion admitted to our hospital from February 2024 to January 2025 were selected and divided into two groups using a double-blind method. The intervention group received low ion coagulation amine technology, while the control group received conventional saline medium method. The results of blood matching tests, coagulation function indicators, blood indicators, and irregular antibody positive test results were compared between the two groups.Results: The main test positive rate, secondary test positive rate, and total positive rate in the intervention group were higher than those in the control group (P<0.05). The two groups showed no statistically significant differences in false positive rate and blood matching time (P>0.05). After 24 h of blood transfusion, both groups showed increases in thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), hematocrit (Hct), hemoglobin (Hb) and platelet count (Plt), and decreases in fibrinogen (FIB) (P<0.05). There were no statistically significant differences in TT, PT, APTT, FIB, Hct, Hb, and Plt between the two groups before and after 24 h of blood transfusion (P>0.05). The main test positive rate, secondary test positive rate and total positive detection rate of irregular antibodies in the intervention group were higher than those in the control group (P<0.05). Conclusion: The low ion coagulation amine technology has a high positive detection rate and has a relatively small impact on coagulation and blood indicators. It has certain convenience and safety, and is worthy of application and promotion.