Objective: To explore the effects of Ankle-three acupuncture combined with isokinetic muscle strength training on ankle joint spasm after stroke. Methods: 114 patients with ankle spasm after stroke in our department from April 2022 to May 2024 were selected as the study objects. 57 patients in the control group were given three ankle needles combined with ordinary rehabilitation training, and 57 patients in the observation group were given three ankle needles combined with isokinetic muscle strength training of lower limb closure chain. Ankle joint function, standing balance function, lower limb motor function and activities of daily living were compared between the two groups. Results: After 8 weeks of training, there was no significant difference between the two groups in the level of passive peak moment and active peak moment (P>0.05). The range of active and passive ankle motion increased in both groups (P<0.05), and was higher in the observation group (P<0.05). The score of ankle plantar flexor muscle and the Angle of foot varus at rest were lower in both groups (P<0.05), and lower in observation group (P<0.05). Muscle strength and Berg balance scale scores were increased in both groups (P<0.05), and higher in observation group (P<0.05). After 8 weeks of training, the step frequency, step speed, step length and touch time of both groups were increased (P<0.05), and the observation group was higher (P<0.05). Barthel index was increased in both groups (P<0.05), and higher in observation group (P<0.05). Conclusion: Ankle-three acupuncture combined with isokinetic muscle strength training can improve ankle joint function and standing balance function, enhance lower limb motor function, and improve the ability of daily living in patients with ankle spasm after stroke.