Abstract Objective: To investigate the mechanism of Wuzi Powder in treating Postoperative pain after hysteroscopy through network pharmacology and molecular docking. Methods: The active components of five herbs in Wuzi Powder were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The target prediction was conducted using the Swiss Target Prediction platform. Disease-related targets associated with Postoperative pain after hysteroscopy were retrieved from databases including GeneCards and OMIM. A protein-protein interaction (PPI) network was constructed using the STRING database. Functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), were conducted using the DAVID bioinformatics resource. Finally, molecular docking was employed to validate the core targets and active components. Results: A total of 20 bioactive components were screened, and 1,582 disease-related targets were identified. Among them, 153 potential therapeutic targets for Postoperative pain after hysteroscopy were determined. The main active components of Wuzi Powder in alleviating this pain were identified as quercetin, 6-hydroxy-luteolin, luteolin, isorhamnetin, and kaempferol. At the same time, the key targets were predicted to be glyceraldehyde-3-phosphate dehydrogenase (GAPDH), AKT serine/threonine-protein kinase 1 (AKT1), signal transducer and activator of transcription 3 (STAT3), epidermal growth factor receptor (EGFR), and caspase 3 (CASP3). The therapeutic effects on Postoperative pain after hysteroscopy were primarily mediated through the regulation of the AGE-RAGE signaling pathway, thereby exerting anti-inflammatory, antioxidant, and analgesic actions. Conclusion: The analgesic effect of Wuzi Powder is mediated through multi-component synergy, multi-target regulation, and multi-pathway integration, preliminarily elucidating its mechanism for managing Postoperative pain after hysteroscopy.