Objective: To investigate the role and mechanism of circular LINC01638 in the growth and invasion of gastric cancer(Gastric Cancer,GC). Methods: qRT-PCR was used to detect the expression of LINC01638 in different gastric cancer cell lines and normal gastric mucosal epithelial cells. The sh-LINC01638 and sh Ctr plasmids were transfected into MGC-803 and AGS cells, respectively,, and the transfection efficiency was detected by qRT-PCR. The effects of LINC01638 on the growth and invasion of gastric cancer cells were detected by CCK-8 and Transwell assays, respectively. Western blot was used to detect the protein expression level of transforming growth factor - β 1 (TGF - β 1). Results: qRT-PCR results showed that the expression level of LINC01638 in gastric cancer cell lines was significantly higher than that in normal gastric mucosal epithelial cells (P<0.05). The expression level of LINC01638 in gastric cancer cells transfected with sh-LINC01638 was significantly downregulated, indicating successful transfection. CCK-8 assay showed that the OD values of MGC-803 and SGC-7901 cells in the sh-LINC01638 group were significantly lower than those in the sh-Ctr group (P<0.05). Transwell assay results showed that the number of MGC-803 and AGS cells that penetrated the matrix gel in the sh-LINC01638 group was significantly lower than that in the sh-Ctr group (P<0.05). Western blot results showed that the relative expression level of TGF-β1 in the sh-LINC01638 group was lower than that in the sh-Ctr group (P<0.05). Conclusion: LINC01638 is highly expressed in gastric cancer. Knocking down the expression of LINC01638 in gastric cancer cells can inhibit cell growth and invasion, and its mechanism may be related to the regulation of TGF-β1.